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KMID : 1098420170250050315
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Korean Journal of Medicinal Crop Science
2017 Volume.25 No. 5 p.315 ~ p.321
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Protective Effects of Glycyrrhiza uralensis Radix Extract and Its Active Compounds on H2O2-induced Apoptosis of C6 Glial Cells
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Park Chan-Hum
Kim Ji-Hyun
Choi Seung-Hak
Shin Yoo-Su
Lee Sang-Won
Cho Eun-Ju
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Abstract
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Background: Glycyrrhiza uralensis Radix (GR) is a crude drugs used in Asian countries that has been reported to prevent the progression of neurodegenerative diseases such as Alzheimer’s disease. The present study examined whether GR and its active compounds, glycyrrhizic acid (GA) and isoliquiritigenin (IL), exerted protective effects on H2O2-induced oxidative damage in C6 glial cells.
Methods and Results: We exposed C6 glial cells to hydrogen peroxide (H2O2) for 24 h and investigated the cellular response to GR and its active compounds by evaluating cell viability, reactivie oxygen species (ROS) production, and apoptosis-related protein expression. GR successfully mitigated the reduced cell viability and ROS production induced by H2O2 in C6 glial cells, IL and GA significantly increased the cell viability and decreased ROS production. In addition, IL and GA down-regulated apoptotic Baxdependent caspase-3 activation, but each compound exerted different mechanisms, i.e., IL dose-dependently decreased ROS production and, GA up-regulated anti-apoptotic Bcl-2 expression.
Conclusions: These results demonstrated that GR and its active components, IL and GA, exhibit potential for use as natural neurodegenerative agents for the modulation of apoptosis in C6 glial cells.
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KEYWORD
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Glycyrrhiza uralensis, Apoptosis, C6 Glial Cell, Glycyrrhizic Acid, Isoliquiritigenin, Oxidative Stress
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